Advanchrom C18: Redefining HPLC Performance Through Innovation
Welch Advanchrom C18 is a high-performance HPLC column meticulously engineered for demanding separation and analysis workflows. Designed to deliver exceptional reliability and precision, Advanchrom C18 sets a new benchmark in chromatographic performance.
Breakthrough in Core Technology
Built upon ultra-pure hybrid silica, Advanchrom C18 enables an extended pH operating range of 1 to 12.5, far surpassing conventional industry standards. Coupled with our unique bonding and end-capping processes, the column maintains outstanding separation performance even under extreme analytical conditions.
Key Advantages of Advanchrom C18
- Wide pH Tolerance
Capable of withstanding mobile phases across pH 1–12.5, this column is particularly well-suited for the separation of basic compounds in complex matrices. It offers flexibility for method development and expands application possibilities in challenging alkaline conditions. - Enhanced Anti-Fouling Properties
Advanchrom C18 significantly reduces the adsorption of non-polar compounds, minimizing impurity retention and column contamination. In scenarios that require high column efficiency, this column reduces maintenance downtime caused by column contamination, greatly improving overall lab efficiency. Ideal for high-throughput testing scenarios such as:- Food safety analysis (e.g., preservatives and additives)
- Traditional Chinese medicine (e.g., crude herbs and decoction pieces)
- Pharmaceutical QC (e.g., reaction monitoring and impurity profiling)
- Exceptional Batch-to-Batch Reproducibility
Thanks to a fully standardized manufacturing process and rigorous quality control, Advanchrom C18 ensures consistency in key performance metrics—retention time, resolution, and plate number—across production batches. This consistency supports seamless method transfer and reliable large-scale testing.
Rigorously Validated Reproducibility
Chromatographic Conditions:
- Column Specification: 4.6×150 mm, 5 µm
- Mobile Phase: acetonitrile/water = 65/35
- Flow Rate: 1.0 mL/min
Analytes:
- Uracil
- Phenol
- 4-Nitrochlorobenzene
- Toluene
| Batch # | Ret. Time | Plates | Tailing | Resolution |
| 1 | 4.574 | 11346 | 1.09 | 4.47 |
| 2 | 4.612 | 11397 | 1.10 | 4.43 |
| 3 | 4.603 | 10921 | 1.07 | 4.39 |
| 4 | 4.589 | 11320 | 1.08 | 4.40 |
| 5 | 4.583 | 11453 | 1.08 | 4.38 |
Five batches of column packing material were validated to deliver consistent retention time, theoretical plates, tailing factors, and resolution, allowing analysts to proceed with confidence, knowing batch variation will not compromise their results.
Proven High-Temperature Endurance
Chromatographic Conditions:
- Column Specification: 4.6×250 mm, 5 µm
- Mobile Phase: acetonitrile/water = 65/35
- Flow Rate: 1.0 mL/min
- Column Temperature: 65 ℃
Analytes:
- Uracil
- Phenol
- 4-Nitrochlorobenzene
- Toluene
Subjected to 65 °C column temperature and 1,000 consecutive injections, Advanchrom C18 demonstrated excellent peak shape and maintained high theoretical plate counts. Even under prolonged high-temperature use, the column performs with remarkable stability, minimizing method interruption and reruns.
Superior High-pH Stability
Chromatographic Conditions:
- Column Specification: 2.1×50 mm, 5 µm
- Mobile Phase: 10 mmol/L ammonium carbonate/methanol = 70/30 (pH=10.0)
- Flow Rate: 0.21 mL/min
- Column Temperature: 50 ℃
- Injection Volume: 0.6 µL
- Wavelength: 254 nm
Analytes:
- Uracil
- Sodium p-toluenesulfonate
- Acetanilide
- Procainamide
Advanchrom C18
Conventional C18, note the peak fronting at 60 hours
After 60 hours of continuous injection under high-pH mobile phase conditions, Advanchrom C18 continued to deliver sharp peaks and high efficiency. Its robust resistance to alkaline degradation ensures reliable long-term performance in harsh conditions.
Brand: Advanchrom
Phase: Adv-C18
Particle Size: 5μm
Pore Size: 180 Å
USP Classification: L1
Separation Mode: Reversed Mode
pH Range: 1.0-12.5
Surface Area: 220 m²/g
Carbon Load: 14%
Max.Pressure: 40MPa
Endcapped: Yes
Max.Temp.: 60°C (pH ≤6.5) and 40°C (pH ＞6.5)
Particle Shape: Spherical
Column Cross Reference:
(Please kindly note that columns among brands are never 100% the same. The below cross reference list indicates alternatives column(s) that will likely give a similar selectivity. Inevitable differences in retention time, resolution and theoretical plate count exist and may affect the results for certain circumstances.)
Advanchrom C18: Redefining HPLC Performance Through Innovation
Welch Advanchrom C18 is a high-performance HPLC column meticulously engineered for demanding separation and analysis workflows. Designed to deliver exceptional reliability and precision, Advanchrom C18 sets a new benchmark in chromatographic performance.
Breakthrough in Core Technology
Built upon ultra-pure hybrid silica, Advanchrom C18 enables an extended pH operating range of 1 to 12.5, far surpassing conventional industry standards. Coupled with our unique bonding and end-capping processes, the column maintains outstanding separation performance even under extreme analytical conditions.
Key Advantages of Advanchrom C18
- Wide pH Tolerance
Capable of withstanding mobile phases across pH 1–12.5, this column is particularly well-suited for the separation of basic compounds in complex matrices. It offers flexibility for method development and expands application possibilities in challenging alkaline conditions. - Enhanced Anti-Fouling Properties
Advanchrom C18 significantly reduces the adsorption of non-polar compounds, minimizing impurity retention and column contamination. In scenarios that require high column efficiency, this column reduces maintenance downtime caused by column contamination, greatly improving overall lab efficiency. Ideal for high-throughput testing scenarios such as:- Food safety analysis (e.g., preservatives and additives)
- Traditional Chinese medicine (e.g., crude herbs and decoction pieces)
- Pharmaceutical QC (e.g., reaction monitoring and impurity profiling)
- Exceptional Batch-to-Batch Reproducibility
Thanks to a fully standardized manufacturing process and rigorous quality control, Advanchrom C18 ensures consistency in key performance metrics—retention time, resolution, and plate number—across production batches. This consistency supports seamless method transfer and reliable large-scale testing.
Rigorously Validated Reproducibility
Chromatographic Conditions:
- Column Specification: 4.6×150 mm, 5 µm
- Mobile Phase: acetonitrile/water = 65/35
- Flow Rate: 1.0 mL/min
Analytes:
- Uracil
- Phenol
- 4-Nitrochlorobenzene
- Toluene
| Batch # | Ret. Time | Plates | Tailing | Resolution |
| 1 | 4.574 | 11346 | 1.09 | 4.47 |
| 2 | 4.612 | 11397 | 1.10 | 4.43 |
| 3 | 4.603 | 10921 | 1.07 | 4.39 |
| 4 | 4.589 | 11320 | 1.08 | 4.40 |
| 5 | 4.583 | 11453 | 1.08 | 4.38 |
Five batches of column packing material were validated to deliver consistent retention time, theoretical plates, tailing factors, and resolution, allowing analysts to proceed with confidence, knowing batch variation will not compromise their results.
Proven High-Temperature Endurance
Chromatographic Conditions:
- Column Specification: 4.6×250 mm, 5 µm
- Mobile Phase: acetonitrile/water = 65/35
- Flow Rate: 1.0 mL/min
- Column Temperature: 65 ℃
Analytes:
- Uracil
- Phenol
- 4-Nitrochlorobenzene
- Toluene
Subjected to 65 °C column temperature and 1,000 consecutive injections, Advanchrom C18 demonstrated excellent peak shape and maintained high theoretical plate counts. Even under prolonged high-temperature use, the column performs with remarkable stability, minimizing method interruption and reruns.
Superior High-pH Stability
Chromatographic Conditions:
- Column Specification: 2.1×50 mm, 5 µm
- Mobile Phase: 10 mmol/L ammonium carbonate/methanol = 70/30 (pH=10.0)
- Flow Rate: 0.21 mL/min
- Column Temperature: 50 ℃
- Injection Volume: 0.6 µL
- Wavelength: 254 nm
Analytes:
- Uracil
- Sodium p-toluenesulfonate
- Acetanilide
- Procainamide
Advanchrom C18
Conventional C18, note the peak fronting at 60 hours
After 60 hours of continuous injection under high-pH mobile phase conditions, Advanchrom C18 continued to deliver sharp peaks and high efficiency. Its robust resistance to alkaline degradation ensures reliable long-term performance in harsh conditions.
Brand: Advanchrom
Phase: Adv-C18
Particle Size: 5μm
Pore Size: 180 Å
USP Classification: L1
Separation Mode: Reversed Mode
pH Range: 1.0-12.5
Surface Area: 220 m²/g
Carbon Load: 14%
Max.Pressure: 40MPa
Endcapped: Yes
Max.Temp.: 60°C (pH ≤6.5) and 40°C (pH ＞6.5)
Particle Shape: Spherical
Column Cross Reference:
(Please kindly note that columns among brands are never 100% the same. The below cross reference list indicates alternatives column(s) that will likely give a similar selectivity. Inevitable differences in retention time, resolution and theoretical plate count exist and may affect the results for certain circumstances.)