Blossmate AA-D

Product Overview

Blossmate AA-D column is prepared by uniformly coating the surface of octadecyl-bonded silica gel with D-penicillamine derivatives. It is based on the mechanism of ligand exchange of copper(II), enabling efficient chiral separation of amino acids, amino acid derivatives, organic acids, among other chiral compounds. It is an ideal choice for the analysis and quality control of chiral amino acids.

Separation Mechanis

Separation is achieved through differences in the coordination interactions between target analytes and copper ions chelated on the chiral stationary phase.

Applicable Samples

It is applicable to chiral compounds capable of chelating with copper ions, including amino acids and their derivatives, chiral dicarboxylic and polycarboxylic acids, chiral diamines and polyamines, peptides, amino alcohols, among other compounds. It is particularly well suited for routine analysis and quality control of chiral amino acids.

Product Features

• No Derivatization Required: Samples can be directly injected and analyzed without pre-column derivatization, simplifying sample preparation, shortening analysis time, and reducing method development costs.
• UV Detection Compatible: After coordination with copper ions, amino acids can be directly detected by UV at 254 nm. This makes Blossmate AA-D columns compatible with conventional HPLC UV detectors, requiring no specialized detection equipment.
• High-Efficiency Chiral Recognition: The D-penicillamine chiral ligand provides excellent enantioselectivity toward amino acids. The measured resolution for proline enantiomers reaches 10.55, while the resolutions of phenylalanine, tyrosine, and other amino acid enantiomers also exceed 5.

Specifications and Use Precautions

Blossmate AA-D is available in two dimensions: the 4.6 × 50 mm, 3 μm short one is suitable for rapid screening, while the 4.6 × 150 mm, 5 μm one provides higher column efficiency and resolution for routine analysis and quality control, balancing analytical speed with separation performance.

When using Blossmate AA-D columns, the corresponding mobile phase is a copper salt solution with an optional organic modifier. The organic modifier can be selected from isopropyl alcohol (isopropanol, preferred choice), methanol, ethanol, and acetonitrile, with a proportion of no large than 15% (30% for methanol). The copper salt is often a high-purity copper sulfate (anhydrous, CuSO₄), with copper ion concentration between 0.5 mmol/L and 4 mmol/L (2 mmol/L is recommended).

Other method parameters for Blossmate AA-D columns:

• pH range: between 4.0 and 7.0.
• Column temperature: 5 °C to 40 °C
• Flow rate: between 0.5 mL/min and 2.0 mL/min
• Maximum temperature tolerance: 30 MPa.