Advanchrom SCX

Advanchrom SCX is a high-performance column designed for protein separation. It is built on advanced monodisperse nonporous polymer microspheres and utilizes a proprietary surface bonding technology. This column is ideal for the separation and characterization of charge variants in monoclonal antibodies, bispecific antibodies, fusion proteins, and antibody–drug conjugates (ADCs).

Features:

• High resolution and outstanding selectivity for charge variants of antibody molecules
• Advanced polymer bead technology, yielding high eciency and excellent batch-to-batch consistency
• Proprietary surface modification, oering minimized non-specific adsorption and high recovery rates

Application Examples

Analysis of NIST mAb Charge Variant

• Column: Advanchrom SCX (4.6×150 mm, 5 μm)
• Mobile Phase: A) 20 mM MES (pH 6.5); B) 20 mM MES (pH 6.5) + 0.5 M NaCl
• Flow Rate: 0.3 mL/min
• Detector Wavelength: 280 nm
• Column Temperature: 30 °C
• Injection Volume: 2 μL
• Sample Concentration: 10 mg/mL
• Gradient:
  

  t (min)	A%	B%
  0	90	10
  3	70	30

Analysis of Rituximab Charge Variant

• Column: Advanchrom SCX (4.6×150 mm, 5 μm)
• Mobile Phase: A) 20 mM MES (pH 6.5); B) 20 mM MES (pH 6.5) + 0.3 M NaCl
• Flow Rate: 0.5 mL/min
• Detector Wavelength: 280 nm
• Column Temperature: 30 °C
• Injection Volume: 10 μL
• Sample Concentration: 1 mg/mL
• Gradient:
  

  t (min)	A%	B%
  0	80	20
  20	65	35
  20.1	0	100
  23	0	100
  23.1	80	20
  30	80	20