Sample Solution(full scale)

ColumnUltisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043)
Detection290nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul

Mobile Phase

Time0.1% Acetic acidMethanol/Acetonitrile=4/1
0-1580-6520-35
15-2565-5535-45
25-3555-5045-50
35-4550-1050-90

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