Sample Solution(full scale)
| Column | Ultisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043) |
| Detection | 290nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
Mobile Phase
| Time | 0.1% Acetic acid | Methanol/Acetonitrile=4/1 |
| 0-15 | 80-65 | 20-35 |
| 15-25 | 65-55 | 35-45 |
| 25-35 | 55-50 | 45-50 |
| 35-45 | 50-10 | 50-90 |
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