P/N: H02755-03100

Ultisil BHS-C18, 10μm, 120Å, 1g

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In the packing and purification processes, the packing material undergoes significant mechanical pressure. Therefore, the mechanical strength requirements for packing materials are generally higher than those for analytical-grade packing materials. The mechanical strength of the packing material is a crucial quality indicator, as it directly influences the lifespan and production process of the material. Frequent packing and unpacking demand exceptionally stable packing materials. Insufficient mechanical strength can lead to packing material breakage, resulting in increased column pressure, decreased column efficiency, and compromised performance. This underscores the need for high mechanical strength and robust bonding techniques to extend the lifespan of packing materials, thereby enhancing customer production efficiency and reducing costs associated with separation purification.

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Pairs well with

Ultisil Series HPLC Columns are based on ultra-pure ( purity > 99.999% ) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development, owing to complete bonding chemistries and stable performance.

Features

  • Ultra-pure spherical porous silica, purity > 99.999%
  • High efficiency: theoretical plate > 80000/m
  • Excellent peak symmetry: tailing factor=0.95~1.05
  • Exceptional lot-to-lot reproducibility

SEM Pictures of Ultisil Particles


Trace Amount Metal Contents Test

A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
Ultisil XB-C18 provides good peak shapes in the separation of these three compounds under pH 7.6, which indicates Ultisil silica contains hardly any metals.

Separation of Basic Compounds Antidepressant at pH 7.0

Comparison of Peak Shape between Batch to Batch

Capacity Factor(K’) of Batch to Batch Reproducibility

Brand: Ultisil

Phase: BHS-C18

Particle Size: 10μm

Pore Size: 120Å

USP Classification:

Separation Mode:

pH Range: 2.0-9.0

Surface Area: 320m2/g

Carbon Load: 19%

Max.Pressure: 40Mpa

Endcapped:

Max.Temp.:

Particle Shape:

Similar Columns:

(Note: The above cross reference indicates alternative column(s) that will likely give a similar selectivity. There is no guarantee of 100% substitution and thus is for your reference only.)

Product Description

Ultisil Series HPLC Columns are based on ultra-pure ( purity > 99.999% ) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development, owing to complete bonding chemistries and stable performance.

Features

  • Ultra-pure spherical porous silica, purity > 99.999%
  • High efficiency: theoretical plate > 80000/m
  • Excellent peak symmetry: tailing factor=0.95~1.05
  • Exceptional lot-to-lot reproducibility

SEM Pictures of Ultisil Particles


Trace Amount Metal Contents Test

A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
Ultisil XB-C18 provides good peak shapes in the separation of these three compounds under pH 7.6, which indicates Ultisil silica contains hardly any metals.

Separation of Basic Compounds Antidepressant at pH 7.0

Comparison of Peak Shape between Batch to Batch

Capacity Factor(K’) of Batch to Batch Reproducibility

Specifications

Brand: Ultisil

Phase: BHS-C18

Particle Size: 10μm

Pore Size: 120Å

USP Classification:

Separation Mode:

pH Range: 2.0-9.0

Surface Area: 320m2/g

Carbon Load: 19%

Max.Pressure: 40Mpa

Endcapped:

Max.Temp.:

Particle Shape:

Similar Columns:

(Note: The above cross reference indicates alternative column(s) that will likely give a similar selectivity. There is no guarantee of 100% substitution and thus is for your reference only.)

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