P/N: H00274-11912

Ultisil DLC UHPLC HILIC Amphion II, 1.8μm, 2.1×100mm (1pcs/pk)

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The diamond coating technology enhances the inner surface of the chromatography column with strong hydrophobicity, which overcomes the adverse effects of the column wall on separation.The high hardness and significant tensile properties of the diamond coating resolve the conflict between column mechanical strength and the influence of metal ions on separation. DLC HPLC Columns are ideal for preparation and purification of biological samples, such as proteins, polysaccharides, and nucleic acids.

Ultisil UHPLC HILIC Amphion II column features a zwitterionic (amphion) structure containing both positively and negatively charged centers. This unique composition significantly enhances the retention of acidic and basic compounds through ion-exchange mechanisms. With excellent hydrophilicity, this stationary phase enables effective HILIC-mode separation of polar, hydrophilic small molecules and basic compounds. Compared to traditional silica- and amino-based HILIC materials, it offers superior reproducibility and more stable HILIC separation performance.

Quantity: 1 pcs/pk

Pairs well with

The Ultimate Experience of DLC Columns

  • Enhancing the inner surface of the chromatography column with strong hydrophobicity overcomes the adverse effects of the column wall on separation.
  • The high hardness and significant tensile properties of the diamond coating resolve the conflict between column mechanical strength and the influence of metal ions on separation.
  • Ideal for preparation and purification of biological samples, such as proteins, polysaccharides, and nucleic acids.

Detection of Bovine Serum Albumin

Sample Solution:A precise amount of Bovine Serum Albumin (BSA) was accurately weighed, dissolved in the mobile phase, and diluted to achieve a sample solution in which every 1mL contained 0.1mg of the sample

Conclusion: The Diamond like carbon column exhibited significantly increased response values.

Detection of Dexamethasone sodium phosphate

      Conclusion: 

      1.The diamond column minimizes the specific adsorption behavior of the chromatographic column tube, achieving efficient separation while showing higher sensitivity and response values.

      2. The peak signal of the diamond column can be increased several times, indicating that it has obvious advantages in testing metal-sensitive compounds.

          Brand: Ultisil

          Phase: HILIC Amphion

          Particle Size: 1.8μm

          Pore Size: 120Å

          USP Classification: L114

          Separation Mode: HILIC Mode

          pH Range: 2.0-8.0

          Surface Area: 320m2/g

          Carbon Load: 5%

          Max.Pressure: 60Mpa

          Endcapped:

          Max.Temp.: 60°C

          Particle Shape: Spherical

          Column Cross Reference:
          (Please kindly note that columns among brands are never 100% the same. The below cross reference list indicates alternatives column(s) that will likely give a similar selectivity. Inevitable differences in retention time, resolution and theoretical plate count exist and may affect the results for certain circumstances.)

          Product Description

          The Ultimate Experience of DLC Columns

          • Enhancing the inner surface of the chromatography column with strong hydrophobicity overcomes the adverse effects of the column wall on separation.
          • The high hardness and significant tensile properties of the diamond coating resolve the conflict between column mechanical strength and the influence of metal ions on separation.
          • Ideal for preparation and purification of biological samples, such as proteins, polysaccharides, and nucleic acids.

          Detection of Bovine Serum Albumin

          Sample Solution:A precise amount of Bovine Serum Albumin (BSA) was accurately weighed, dissolved in the mobile phase, and diluted to achieve a sample solution in which every 1mL contained 0.1mg of the sample

          Conclusion: The Diamond like carbon column exhibited significantly increased response values.

          Detection of Dexamethasone sodium phosphate

              Conclusion: 

              1.The diamond column minimizes the specific adsorption behavior of the chromatographic column tube, achieving efficient separation while showing higher sensitivity and response values.

              2. The peak signal of the diamond column can be increased several times, indicating that it has obvious advantages in testing metal-sensitive compounds.

                  Specifications

                  Brand: Ultisil

                  Phase: HILIC Amphion

                  Particle Size: 1.8μm

                  Pore Size: 120Å

                  USP Classification: L114

                  Separation Mode: HILIC Mode

                  pH Range: 2.0-8.0

                  Surface Area: 320m2/g

                  Carbon Load: 5%

                  Max.Pressure: 60Mpa

                  Endcapped:

                  Max.Temp.: 60°C

                  Particle Shape: Spherical

                  Column Cross Reference:
                  (Please kindly note that columns among brands are never 100% the same. The below cross reference list indicates alternatives column(s) that will likely give a similar selectivity. Inevitable differences in retention time, resolution and theoretical plate count exist and may affect the results for certain circumstances.)

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