Ultisil Series HPLC Columns are based on ultra-pure (purity > 99.999%) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development. owing to complete bonding chemistries and stable performance
Features
- Ultra-pure spherical porous silica, purity > 99.999%
- Wide pH range: 2.0-8.0
- High efficiency: theoretical plate > 80000/m
- Excellent peak symmetry: tailing factor=0.95~1.05
- Exceptional lot-to-lot reproducibility
Ultisil HPLC Column Packing Materials
Trace Amount Metal Contents Test
A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
|
Column: |
Ultisil® XB-C18, 4.6 ×150 mm, 5 μm |
|
Mobile Phase |
20 mM phosphate(pH 7.6) / methanol=55/45 |
|
Flow Rate |
1.0 mL/min |
|
Detector |
215 nm |
|
Temperature: |
25°C |
|
Injection Volume |
1 μL |
|
Samples: |
1) 4,4’-Dipyridyl 2) 2,2’-Dipyridyl 3) 1,2-Dihydroxylnaphthalene |
Conclusion: Ultisi XB-C18 provides good peak shapes in the separation of these three compounds under pH 7.6, which indicates Ultisil silica contains hardly any metals.
Brand: Ultisil
Phase: HILIC Silica
Particle Size: 3µm
Pore Size: 120Å
USP Classification: L3
Separation Mode: HILIC Mode
pH Range: 2.0-8.0
Surface Area: 320m2/g
Carbon Load:
Max.Pressure: 60Mpa
Endcapped:
Max.Temp.: 60°C(pH≤6.5) and 40°C(pH>6.5)
Particle Shape: Spherical
Similar Columns:
(Please kindly note that columns among brands are never 100% the same. The below cross reference list indicates alternatives column(s) that will likely give a similar selectivity. Inevitable differences in retention time, resolution and theoretical plate count exist and may affect the results for certain circumstances. Click here for a more up-to-date cross-reference tool.)
Ultisil Series HPLC Columns are based on ultra-pure (purity > 99.999%) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development. owing to complete bonding chemistries and stable performance
Features
- Ultra-pure spherical porous silica, purity > 99.999%
- Wide pH range: 2.0-8.0
- High efficiency: theoretical plate > 80000/m
- Excellent peak symmetry: tailing factor=0.95~1.05
- Exceptional lot-to-lot reproducibility
Ultisil HPLC Column Packing Materials
Trace Amount Metal Contents Test
A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
|
Column: |
Ultisil® XB-C18, 4.6 ×150 mm, 5 μm |
|
Mobile Phase |
20 mM phosphate(pH 7.6) / methanol=55/45 |
|
Flow Rate |
1.0 mL/min |
|
Detector |
215 nm |
|
Temperature: |
25°C |
|
Injection Volume |
1 μL |
|
Samples: |
1) 4,4’-Dipyridyl 2) 2,2’-Dipyridyl 3) 1,2-Dihydroxylnaphthalene |
Conclusion: Ultisi XB-C18 provides good peak shapes in the separation of these three compounds under pH 7.6, which indicates Ultisil silica contains hardly any metals.
Brand: Ultisil
Phase: HILIC Silica
Particle Size: 3µm
Pore Size: 120Å
USP Classification: L3
Separation Mode: HILIC Mode
pH Range: 2.0-8.0
Surface Area: 320m2/g
Carbon Load:
Max.Pressure: 60Mpa
Endcapped:
Max.Temp.: 60°C(pH≤6.5) and 40°C(pH>6.5)
Particle Shape: Spherical
Similar Columns:
(Please kindly note that columns among brands are never 100% the same. The below cross reference list indicates alternatives column(s) that will likely give a similar selectivity. Inevitable differences in retention time, resolution and theoretical plate count exist and may affect the results for certain circumstances. Click here for a more up-to-date cross-reference tool.)