Solution of sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 5 | 100 | 0 |
| 20 | 49 | 51 |
| 30 | 49 | 51 |
| Column | Welch Ultisil® Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | A：Acetonitrile-methanol-0.1% acetic acid solution (5:35:60)； B：Acetonitrile-methanol-0.1% acetic acid solution (5:80:15) |
| Detector | 250nm |
| Temperature | 50℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
If you have any problem or require further information, please contact info@welchmat.com.
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