HPLC Ultisil®Plus C18 phosphoric acid water, acetonitrile
Test Report
Chromatography conditions:
Column |
Welch Ultisil®Plus C18（4.6×250mm,5μm） |
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Mobile Phase |
A:0.1% phosphoric acid water B:acetonitrile |
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Column Temperature |
Room temperature |
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Detector |
UV |
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Injection Volume |
10μL |
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Flow Rate |
0.8 ml/min |
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Detect wavelength |
254nm |
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Elution procedure |
|
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Note |
/ |
Chromatogram and Data:
1) Blank analysis spectrum:
Retention time (min) |
Peak Area(mAU*min) |
Peak height（mAU) |
Relative peak area(%) |
Number of plates(EP) |
Resolution(EP) |
Sum |
|
0.000 |
0.000 |
0.000 |
0.00 |
2) sst sample analysis pattern
Name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height（mAU) |
Relative peak area(%) |
Number of plates(EP) |
Resolution(EP) |
Main substance |
6.704 |
6.284 |
49.665 |
65.99 |
17253 |
1.80 |
7.074 |
0.222 |
1.740 |
2.33 |
18513 |
1.71 |
|
7.448 |
3.017 |
21.054 |
31.68 |
16519 |
N.a |
|
|
Sum |
|
9.523 |
72.460 |
100.00 |
52285.00 |
Conclusion: