Etibatide on Ultisil Polar RP
Gradient Program
| Time（min） | A% | B% |
| 0 | 85 | 15 |
| 10 | 85 | 15 |
| 30 | 70 | 30 |
| 40 | 60 | 40 |
| 50 | 45 | 55 |
| 60 | 15 | 85 |
| 60.1 | 85 | 15 |
| 70 | 85 | 15 |
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | Mobile phase A: 0.05mol/L potassium dihydrogen phosphate (pH adjusted to 2.27 with phosphate) Mobile phase B: 50% acetonitrile water |
| Detector | 220nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |