Gradient Program

Time(min)A%B%
08515
108515
307030
406040
504555
601585
60.18515
708515
ColumnWelch Ultisil Polar RP 4.6×250,5μm (P/N: 00215-31043)
Mobile PhaseMobile phase A: 0.05mol/L potassium dihydrogen phosphate (pH adjusted to 2.27 with phosphate) Mobile phase B: 50% acetonitrile water
Detector220nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul

If you have any problem or require further information, please contact info@welchmat.com.


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