Column: Ultisil XB-C18, 3.0×100mm, 1.8um

1.1. Reference Solution

(Flow Rate: 0.425ml/min; Injection Volume: 2.8ul; Initial Pressure: 427bar)

No.ComponentRt (min)Half Width (min)TypeResolution (EP)Asymmetry (EP)Plates (EP)
Mobile PhaseMethanol : Buffer = 70 : 30, adjust pH to 5.7 with 10% phosphoric acid
Detection215 nm
NotesColumn temperature shall be 30℃; Adjust the mobile phase pH after it’s well mixed.

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