Column: Ultisil XB-C18, 3.0×100mm, 1.8um
1.1. Reference Solution
(Flow Rate: 0.425ml/min; Injection Volume: 2.8ul; Initial Pressure: 427bar)
| No. | Component | Rt (min) | Half Width (min) | Type | Resolution (EP) | Asymmetry (EP) | Plates (EP) |
| 1 | Clotrimazole | 11.208 | 0.188 | M | n.a. | 1.09 | 19588 |
| Mobile Phase | Methanol : Buffer = 70 : 30, adjust pH to 5.7 with 10% phosphoric acid |
| Detection | 215 nm |
| Temperature | 30℃ |
| Notes | Column temperature shall be 30℃; Adjust the mobile phase pH after it’s well mixed. |
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