Wave Length 254mm
| Column | Ultisil® AQ-C18 4.6×250 mm, 3 μm P/N: 00207-21043 |
| Detection | 230, 254 nm |
| Temperature | 40 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: 1.58g ammonium formate dissolve in 320mL water, 160 mL Methanol, 20mL acetonitrile
B: 1.58g ammonium formate dissolve in 1600mL water, 340mL acetonitrile
| Time | A | B |
| 0 | 100 | 0 |
| 2 | 100 | 0 |
| 25 | 40 | 60 |
| 30 | 40 | 60 |
If you have any problem or require further information, please contact info@welchmat.com.
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