Test sample of related substance

ColumnWelch Ultisil Polar RP 4.6×250mm,5μm (P/N: 00215-31043)
Mobile PhaseMethanol-water (measure 0.3 ml phosphoric acid and 0.94 g sodium hexanesulfonate, then dissolve with 1000 ml water)= 240:760;
Detector220nm
Temperature30℃
Flow Rate2 ml/min
Injection Volume20ul

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