| Column | Ultisil® AQ-C18 4.6×200 mm, 5 μm P/N: 00207-31042 |
| Mobile Phase | (Water: Acetonitrile: 1M acetic acid: Solution A=876:120:2.8:1.2): Acetonitrile=93:7 Solution A:14mL triethylamine and 5.7mL glacial acetic acid dilute into 1000mL with Water |
| Detection | 254 nm |
| Temperature | 40 ℃ |
| Flow Rate | 2.5 ml/min |
| Injection Volume | 10 µl |
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