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Cefalexin Related Substance on Ultisil AQ-C18

Related Substance System Suitability Test

Column Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043
Detection 220 nm
Temperature 30 ℃
Flow Rate 1 ml/min
Injection Volume 5 µl

Mobile Phase

A: 0.2mol/L sodium dihydrogen phosphate (Adjust pH to 5.0 with NaOH) B: Methanol

Time A% B%
0 98 2
1 98 2
20 70 30
23 98 2
30 98 2