Determination of 9 anti-allergic drugs including doxylamine in cosmetics
Test Report
Chromatography conditions:
Column |
Welch UItisil® UHPLC AQ-C18 2.1×100，1.8μm |
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Mobile Phase |
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Column Temperature |
30℃ |
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Injection Volume |
5μL |
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Flow Rate |
0.3 ml/min |
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Note |
This test uses the matrix curve, which is recommended to be processed at the same time as the sample. |
Chromatogram and Data:
1) Blank
- 2) Sample solution:
- (3) 100 ng/mL matrix curve
- (4) Sample spiked, 100 ng/mL.
Conclusion:
Using Welchrom® P-SCX solid phase extraction column (60 mg/3 mL), the spiked recovery rates of Desloratadine, Mebhydrolin, Loratadine, Brompheniramine, Pheniramine, Cyclizine, Diphenhydramine, Hydroxyzine, and Chlorpheniramine were 95.3%, 95.6%, 94.3%, 89.3%, 93.3%, 88.3%, 90.7%, 97.3%, and 69.6% respectively. The column spiked recovery rates meet the requirements.
Using Welchrom® P-SCX solid phase extraction column (60 mg/3 mL), the recovery rates of Desloratadine, Mebhydrolin, Loratadine, Brompheniramine, Pheniramine, Cyclizine, Diphenhydramine, Hydroxyzine, and Chlorpheniramine in cosmetics were 103.7%, 97.1%, 100.0%, 98.6%, 95.2%, 95.5%, 95.5%, 104.3%, and 73.7% respectively. The sample recovery rates meet the requirements.