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Carbocisteine on Ultisil AQ-C18

Raw material

Column Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043
Mobile Phase 6.8g monopotassium phosphate, 0.5g Sodium pentanesulfonate dissolved in 1000mL water, adjust pH to 2.5 with Phosphoric Acid
Detection 215 nm
Temperature 35 ℃
Flow Rate 1.0 ml/min
Injection Volume 20 µl
Note Transition with 100%water before use and back flush after use