Berberine Hydrochloride Related Substances on Ultisil LP-C18
System adaptability chart
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Mobile Phase | 0.01 mol/L ammonium dihydrogen phosphate solution (Adjust pH to 2.8 with phosphoric acid): -acetonitrile = 75:25 |
| Detector | 345 nm |
| Temperature | Room Temperature（22℃） |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10µl |
| Notes | Resolution affected by temperature |