Content determination of test sample

ColumnWelch Ultisil Polar RP 4.6×250mm,5μm (P/N: 00215-31043)
Mobile Phase0.05 mol / L potassium dihydrogen phosphate solution(adjust pH to 8.2 with 20% phosphoric acid solution) :Acetonitrile = 45:55
Detector210nm
Temperature30℃
Flow Rate1 ml/min
Injection Volume50ul

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