ColumnWelch Ultisil PFP 4.6×250mm,5μm (P/N: 00224-31043)
  Mobile PhaseA: 0.045 M ammonium formate 0.005 M ammonium acetate: THF = 993.231: 6.769, B: acetonitrile: THF = 963.6: 18.2; % B = 31.1 (0-15 min), 44.6 (20-25 min), 78.4 (50-55 min)
Detector246nm
Temperature40℃
Flow Rate1.1 ml/min
Injection Volume20µl

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