| Column | Welch Ultisil PFP 4.6×250mm，5μm (P/N: 00224-31043) |
| Mobile Phase | A: 0.045 M ammonium formate 0.005 M ammonium acetate: THF = 993.231: 6.769, B: acetonitrile: THF = 963.6: 18.2; % B = 31.1 (0-15 min), 44.6 (20-25 min), 78.4 (50-55 min) |
| Detector | 246nm |
| Temperature | 40℃ |
| Flow Rate | 1.1 ml/min |
| Injection Volume | 20µl |
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