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Anthocyanin on Ultisil PFP(b)

Mixed standard solution

Gradient Program

Time(min) %A %B
0 92 8
5 90 10
22 90 10
30 85 15
35 80 20
40 65 35
45 50 50
50 35 65
55 30 70
55.1 92 8
65 92 8
Column Welch Ultisil PFP 4.6×250mm,5μm (P/N: 00224-31043)
Mobile Phase Mobile phase A: 5.0% formic acid in water Mobile phase B: methanol
Detector 530nm
Temperature 30℃
Flow Rate 0.8 ml/min
Injection Volume 10µl