Amikacin Sulfate on Ultisil Plus C18

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Content ( Isocratic Elution)

Gradient Program (Related Substance)

Time(min)A%B%
06238
306238
600100
700100
716238
806238
ColumnWelch Ultisil Plus C18 4.6×250mm,5μm (P/N: 00260-31043)
  Mobile PhaseA:0.2mol/L phosphate buffer(PH3.0) B:  0.2 mol/L potassium dihydrogen phosphate solution, adjust pH to 3.0 with 0.2 mol/L phosphoric acid
Isocratic Elution (Content Determination)A:B=58:42
Detector200nm
Temperature40℃
Flow Rate1.3 ml/min
Injection Volume10µl
NotesPeak of related substance appears at 26 min;Peak  of content appears at 25 min.

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