3 phosphoric acid sodium on Ultisil AQ-C18
Blank Solution
| Column | Ultisil® AQ-C18, 5μm, 4.6×250mm P/N: 00207-31043 |
| Mobile Phase | 0.2mol/L phosphate buffer (35.8g disodium hydrogen phosphate, 13.6g monopotassium phosphate, add 900mL water, adjust pH to 7.0 with 1mol/L NaOH, 1.61g tetrabutylammonium bromide. Add water to 1000mL) |
| Detection | 271 nm |
| Temperature | 35 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 µl |
| Note | / |