3 phosphoric acid sodium on Ultisil AQ-C18

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Blank Solution

ColumnUltisil® AQ-C18, 5μm, 4.6×250mm    P/N: 00207-31043
Mobile Phase0.2mol/L phosphate buffer (35.8g disodium hydrogen phosphate, 13.6g monopotassium phosphate, add 900mL water, adjust pH to 7.0 with 1mol/L NaOH, 1.61g tetrabutylammonium bromide. Add water to 1000mL)
Detection271 nm
Temperature35 ℃
Flow Rate1.0 ml/min
Injection Volume10 µl
Note/

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