News
-
Review of Webinar: HPLC Method Development and Validation
About Webinar Last friday, Webinar about HPLC method development and validation was held online. Webinar Content a. Tradition strategy for HPLC method development and a survey of automated tools and... -
If pH<2, pay attention to the selection of column
Many analysts will adjust the peak shape and retention time by changing pH during the method development. Because the change of pH will exert various influences on chromatographic behavior of... -
Aging of Column
As is known to us all, in the process of gas phase analysis, column is the "heart" of gas chromatograph, and it plays a crucial role in the qualitative and... -
Autumn is coming, please put on clothes for your column
When many analysts operate liquid chromatogram, columns work at room temperature. If the temperature in the laboratory can be kept constant, problem barely occurs. However, most working places’ temperature constantly... -
Flushing and Regeneration of Column Based on Polymer
Introduction of Column Based on Polymer Polymer matrix columns (Sugar-H, Sugar-Ca) may be contaminated, resulting in decreased column efficiency. Thus, flushing and regeneration are required. However, when flushing these columns... -
Poor Peak Shape, and How to Improve It
When the injection volume of test substance within the range of the security, changing injection volume will affect the peak height and peak area accordingly. But the influence of retention... -
How to Select a Suitable Column?
When you look up the pharmacopoeia method, you may find that it only stipulates the bonding phase of column and the information of other columns is not available. Therefore, you... -
Methods for Prolonging the Column Lifetime
Your column may suffer from short working life, which not only causes rising costs, but also impacts the experimental results. Today, we invite Welch engineers to answer questions about how... -
Gradient Optimization of Liquid Chromatography
In liquid chromatography, for samples with complex components, it is often difficult to use a single chromatographic system to achieve ideal separation effect. Either the separation time is too long,... -
Gel Purification Chromatography
Gel Purification Chromatography Gel chromatography is a separation and analysis technology developed in the early 1960s. According to whether the separated objects are water-soluble substances, gel chromatography can be divided... -
Flushing and Regeneration of Reversed-Phase Column
Do you know the maintenance methods of Welch columns? Some people may use the wrong way to flush columns unconsciously, so it is recommended to learn some practical methods for... -
How to Maintain Our Non-Reversed Phase Columns?
Pollution, loss and other problems will inevitably appear during the use of HPLC columns, thus it is necessary to flush column for maintenance at that time. From the point of...