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If pH<2, pay attention to the selection of column
Many analysts will adjust the peak shape and retention time by changing pH during the method development. Because the change of pH will exert various influences on chromatographic behavior of... -
How to Select a Suitable Column?
When you look up the pharmacopoeia method, you may find that it only stipulates the bonding phase of column and the information of other columns is not available. Therefore, you... -
Determination of Vitamin K1 in Food
Range of Application and Principle Extract vitamin K1 from low fat plant samples such as fruits and vegetables with isopropanol and n-hexane. Purify Vitamin K1 by prep column and remove... -
Gel Purification Chromatography
Gel Purification Chromatography Gel chromatography is a separation and analysis technology developed in the early 1960s. According to whether the separated objects are water-soluble substances, gel chromatography can be divided... -
How Can Polar Compounds be Better Retained?
Reversed-phase columns usually take silica gel, organic/ inorganic hybrid silica gel or polymer as the matrix, while C18 as the bonded phase. Based on the hydrophobic interaction between analytes and... -
Distinction of Related Concepts About Liquid Chromatography
High performance liquid chromatography (HPLC) is a fundamental analytical discipline with many similar concepts. Therefore, it’s necessary for us to learn how to distinguish some easily-confused concepts of liquid chromatography.... -
How to Distinguish Noise from Drift?
During the analysis, some people may encounter the problem that the noise of baseline suddenly gets louder and even baseline drift occurs after long time running. Why and how does... -
The principle of 100% water compatible column
There are many kinds of C18 columns in the market, some are 100% water compatible, some are not. Why can they be used at 100% water? How to distinguish? What... -
Chiral Separation of Remdesivir Isomers
2019-nCoV corona virus pandemic is exploding all over the world, how to cure it has become the most important and urgent matter at present. Remdesivir, developed by Gilead, which is... -
How to distinguish Guard column and Pre-column Inline Filter?
Guard column and Pre-column inline filter are commonly used in LC experiments. But a lot of users are confused about the two products as they look similar. How to distinguish?... -
Why is there always a "ghost" in your chromatogram?
What’s Ghost Peaks? In the chromatographic separation, there are some peaks which appears erratically like ghosts during gradient elution or long-period operation, we call them Ghost Peaks. The harm of... -
C18 under 100% water phase? No problem!
C18 is retentive to non-polar compounds as the non-polarity of C18 group, but for polar compounds, sometimes the mobile phase with high water ratio should be used to make the...