
Column | Xtimate®C18(4.6×250mm,5μm) |
Mobile Phase | 0.02mol/L phosphate buffer (adjust pH to 8.0 with phosphoric acid)/methanol=95/5 |
Detection | 214 nm |
Temperature | 30 ℃ |
Flow Rate | 0.8 ml/min |
Injection Volume | 20 μL |
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