| Column | Ultisil® Diol (4.6×250 mm,5 μm) |
| Mobile Phase | n-hexane:isopropanol:10% glacial acetic acid=240:300:30 (add triethylamine to every 100ml mobile phase 0.05 mL), mixed with glacial acetic acid to adjust the pH to 5. |
| Detection | ELSD drift tube temperature: 90°C air flow: 3.5 bar |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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