Overview

Ion exchange chromatography is a separation method based on the different charges of the protein under certain pH conditions. Due to most of biological molecules with acidic or alkaline groups, anion exchange matrix combined with the negatively charged proteins and cation exchange matrix binds positively charged proteins, and its charged properties and quantity can be changed by adjusting the pH of buffer solution, so that the weak binding can be eluted first, and the strong binding can be eluted later, so as to achieve the purpose of separation and purification.

Today we will introduce Q Tanrose 6FF which is a strong anion exchange medium

Basic Technical Parameters:

Name	Q Tanrose 6FF
Properties	Strong anion exchange packing
Matrix Type	6% agarose
Avg Paticle size & range	90μm，45-165μm
Ligand Density	180-250μmol Cl-/ml
Functional Group	Quaternary amino
Work Flow Rate	50-300cm/h
ph Stability	2-12（long-term), 2-14(short-term）
Chemical stability	2m NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6m guanidine hydrochloride, 8m urea
Storage solution and temprature	20% ethanol, 4-30℃

Applications

Column	Precot Q 6FF 5ml
Sample	2mg/ml β -lactoglobulin
Equilibrium buffer	20mM Sodium acetate，pH=7.4
Eluting buffer	20mM Sodium acetate，1M NaCl, pH=7.4

Figure: β -Lactoglobulin elution peak

Points for Attention

1. The maximum operating pressure shall not exceed 0.3mpa;

2. Avoid using oxidants, anionic detergents and anionic buffers;

3. It is recommended to use 0.22μm or 0.45μm filter membrane for water and buffer before use;

4. Samples are recommended to be centrifuged or filtered with 0.22μm or 0.45μm membrane before loading to reduce impurities, improve protein purification efficiency and prevent from blocking the column

Ordering information and related products

1.Q Tanrose 6FF

No	Specifications
00062-23001	25mL
00062-23002	100mL
00062-23003	500ml
00062-23004	1L
00062-23005	5L

2.PreCot Q 6FF

No	Specification
00062-23011	1mL
00062-23012	5mL

If you have any problem or require further information, please contact info@welchmat.com.