What is “Q” in protein purification product “Q Tanrose FF”?

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Overview

Ion exchange chromatography is a separation method based on the different charges of the protein under certain pH conditions. Due to most of biological molecules with acidic or alkaline groups, anion exchange matrix combined with the negatively charged proteins and cation exchange matrix binds positively charged proteins, and its charged properties and quantity can be changed by adjusting the pH of buffer solution, so that the weak binding can be eluted first, and the strong binding can be eluted later, so as to achieve the purpose of separation and purification.

Today we will introduce Q Tanrose 6FF which is a strong anion exchange medium

Basic Technical Parameters:

NameQ Tanrose 6FF
PropertiesStrong anion exchange packing
Matrix Type6% agarose
Avg Paticle size & range90μm,45-165μm
Ligand Density180-250μmol Cl-/ml
Functional GroupQuaternary amino
Work Flow Rate50-300cm/h
ph Stability2-12(long-term), 2-14(short-term)
Chemical stability2m NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6m guanidine hydrochloride, 8m urea
Storage solution and temprature20% ethanol, 4-30℃

Applications

ColumnPrecot Q 6FF 5ml
Sample2mg/ml β -lactoglobulin
Equilibrium buffer20mM Sodium acetate,pH=7.4
Eluting buffer20mM Sodium acetate,1M NaCl, pH=7.4

Figure: β -Lactoglobulin elution peak

Points for Attention

1. The maximum operating pressure shall not exceed 0.3mpa;

2. Avoid using oxidants, anionic detergents and anionic buffers;

3. It is recommended to use 0.22μm or 0.45μm filter membrane for water and buffer before use;

4. Samples are recommended to be centrifuged or filtered with 0.22μm or 0.45μm membrane before loading to reduce impurities, improve protein purification efficiency and prevent from blocking the column

Ordering information and related products

1.Q Tanrose 6FF

NoSpecifications
00062-2300125mL
00062-23002100mL
00062-23003500ml
00062-230041L
00062-230055L

2.PreCot Q 6FF

NoSpecification
00062-230111mL
00062-230125mL




If you have any problem or require further information, please contact info@welchmat.com.

Categories: News

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