Welchrom ® Alumina-B SPE column

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Betaine is an alkaline substance, mainly cardiotonic glycosides and other steroids. It can be extracted from the roots, stems, leaves and fruits of natural plants or chemically synthesized from trimethylamine and chloroacetic acid.
Betaine is one of the main alkaloids in the fruits, leaves and stems of Lycium barbarum. Its scientific name is trimethylaminoethyllactone. Betaine is contained in the fruits, root bark and leaves of many Lycium plants. The effect of Lycium barbarum on lipid metabolism or anti fatty liver is mainly caused by betaine. A number of research results show that the betaine content in Lycium barbarum leaves is higher than that of other salt tolerant plants.

Welch Materials Inc, has developed a new generation of Welchrom®Alumina-B SPE column according to the content determination method of betaine in Lycium berry varieties in the Chinese Pharmacopoeia in 2020 edition.

Summery

Alumina-B SPE column was applied to the determination of betaine in Lycium barbarum. The method has the advantages of fast speed, simple operation and high accuracy

Principle

Alumina-B SPE column can specifically purify betaine in the sample. The betaine in the sample is extracted by extractant, and the extract is purified by Alumina-B SPE column, in which the impurities are adsorbed on the small column, concentrated and re dissolved after eluting the target, and finally injected into HPLC for determination.

Purification procedure

Alumina-B SPE column activation

sample loading

Elution

Concentration

Re-dissolution

Chromatographic conditions

Chromatographic column: Ultisil®Hilic NH2 4.6×250mm, 5μm.
Mobile phase: Acetonitrile: water=85:15;
Flow rate:1.0mL/min;
Column temperature: 30℃;
Injection volume:10μL;
Detection wavelength: 195nm.

Sample test results

Figure 1. The 0.17mg/mL graph of betaine reference substance
Figure 2. Sample of Lycium barbarum

If you have any problem or require further information, please contact info@welchmat.com.

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