Betaine is an alkaline substance, mainly cardiotonic glycosides and other steroids. It can be extracted from the roots, stems, leaves and fruits of natural plants or chemically synthesized from trimethylamine and chloroacetic acid.
Betaine is one of the main alkaloids in the fruits, leaves and stems of Lycium barbarum. Its scientific name is trimethylaminoethyllactone. Betaine is contained in the fruits, root bark and leaves of many Lycium plants. The effect of Lycium barbarum on lipid metabolism or anti fatty liver is mainly caused by betaine. A number of research results show that the betaine content in Lycium barbarum leaves is higher than that of other salt tolerant plants.
Welch Materials Inc, has developed a new generation of Welchrom®Alumina-B SPE column according to the content determination method of betaine in Lycium berry varieties in the Chinese Pharmacopoeia in 2020 edition.
Summery
Alumina-B SPE column was applied to the determination of betaine in Lycium barbarum. The method has the advantages of fast speed, simple operation and high accuracy
Principle
Alumina-B SPE column can specifically purify betaine in the sample. The betaine in the sample is extracted by extractant, and the extract is purified by Alumina-B SPE column, in which the impurities are adsorbed on the small column, concentrated and re dissolved after eluting the target, and finally injected into HPLC for determination.
Purification procedure
Alumina-B SPE column activation
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sample loading
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Elution
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Concentration
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Re-dissolution
Chromatographic conditions
| Chromatographic column: | Ultisil®Hilic NH2 4.6×250mm, 5μm. |
| Mobile phase: | Acetonitrile: water=85:15; |
| Flow rate: | 1.0mL/min; |
| Column temperature: | 30℃; |
| Injection volume: | 10μL; |
| Detection wavelength: | 195nm. |
Sample test results
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