In today’s era of rapid technological advancement, both in work and in daily life, there is a constant pursuit of speed and efficiency. In the field of analysis and testing, there is an increasing demand for shorter experimental times to achieve faster research and development, higher on-time delivery rates, and increased productivity per individual. Especially for cutting-edge pharmaceutical research organizations, universities, and third-party testing agencies with stringent delivery requirements, they prioritize the use of ultra-high-performance liquid chromatography (UHPLC) for testing, which can provide results 3 to 4 times faster than conventional liquid phase testing.
However, despite having invested in such an expensive UHPLC chromatography system, we have discovered that there are very few options available for UHPLC columns. How can we proceed under such circumstances?
Column problem? Welch Materials has got you covered!
The pillar problem, solved by Welch!
After successfully launching 10 types of UHPLC bonded phases (*see endnote for details), Welch Materials is now introducing two new bonded phases: Amide (polyacrylamide) and HILIC Amphion II (zwitterionic). With our advanced manufacturing technology, we continue to expand our product offerings and provide solutions for your chromatography needs.
These two new key combinations will address the following issues for you:
● Enable fast and efficient separation of sugars, peptides, and low-molecular-weight polar drugs in HILIC mode!
● Enable rapid separation of most polar compounds in HILIC mode without the need for ion-pair reagents!
Introduction to Ultisil® UHPLC Amide Column
Product Features:
- Utilizes a silica-based packing material with bonded polyacrylamide functional groups as the stationary phase, making it particularly suitable for the separation of hydrophilic compounds.
- Provides excellent retention for polar compounds of medium to small molecular size.
- Demonstrates outstanding chemical stability.
- Well-suited for LC/MS analysis of water-soluble polar compounds.
Column Performance Parameters:
Column: Ultisil® UHPLC Amide.
Bonding Phase: Polyacrylamide.
Particle Size: 1.8 μm.
pH Range: 2.0-8.0.
Carbon Loading: 6%.
Pore Size: 120 Å.
Specific Surface Area: 320 m²/g.
Maximum Temperature: 60°C.
Maximum Pressure: 60 MPa.
Application Case:
Analyte: Methionine
Chromatographic Conditions:
Column: Ultimate® UHPLC Amide, 1.8 μm, 2.1 × 100 mm.
Mobile Phase: Acetonitrile/25 mM monosodium phosphate, pH 4.87 (75/25).
Detection Wavelength: 210 nm.
Column Temperature: 35°C.
Flow Rate: 0.2 mL/min.
Injection Volume: 1.0 μL.
Sample Preparation: Dissolve the sample in the mobile phase to prepare a concentration of 2 mg/mL.
| Peak name | Retention time(min) | Peak area(mAU*min) | Peak height(mAU) | Asymmetry | Number of plates | Resolution |
| Methionine | 1.75 | 65.14 | 784.06 | 1.47 | 3130 | n.a |
Introduction to Ultisil® UHPLC HILIC Amphion Ⅱ Column:
Ultisil® UHPLC HILIC Amphion Ⅱ is a new type of dual ion-bonded silica-based HILIC chromatography product developed by MoonX Technology. It is suitable for the separation of most polar compounds and typically utilizes acetonitrile and water as the mobile phase without the need for ion-pair reagents.
Ultisil® UHPLC HILIC Amphion Ⅱ packing material features a structure that contains both cationic and anionic groups, possessing positive and negative charge centers. As a result, it can greatly enhance the retention of acidic and basic compounds through ion-exchange mechanisms. This chromatographic packing material exhibits excellent hydrophilicity, enabling the separation of polar, hydrophilic small-molecule targets, as well as alkaline compounds, in HILIC mode. Compared to traditional silica and amino-based HILIC fillers, this filler provides better reproducibility and a more stable HILIC separation capability.
Product Features:
- Utilizes a dual ion-bonded silica gel stationary phase.
- Enhanced hydrophilic interactions, resulting in strong retention for polar and hydrophilic compounds.
- The filler contains both cationic and anionic groups, providing ion-exchange mechanisms and offering different selectivity compared to regular HILIC fillers.
- Does not require the use of ion-pair reagents, achieving the separation of polar target compounds with a simple mobile phase (typically acetonitrile and water).
Column Performance Parameters:
Column: Ultisil® UHPLC HILIC Amphion Ⅱ.
Bonding Phase: Dual ion-bonded.
Particle Size: 1.8 μm.
pH Range: 2.0-8.0.
Carbon Loading: 5%.
Pore Size: 120 Å.
Specific Surface Area: 320 m²/g.
Maximum Temperature: 60°C.
Maximum Pressure: 60 MPa.
Application Case:
Analyte: Caffeine
Chromatographic Conditions:
Column: Ultisil® UHPLC HILIC Amphion Ⅱ, 1.8 μm, 2.1 × 100 mm.
Mobile Phase: Acetonitrile/Water = 90/10.
Detection Wavelength: 254 nm.
Column Temperature: 30°C.
Flow Rate: 0.2 mL/min.
Injection Volume: 2.0 μL.
Sample Preparation: Dissolve the sample in the mobile phase to prepare a concentration of 0.5 mg/mL.
| Peak name | Retention time(min) | Peak area(%) | Peak height(mAU) | Area(mAU*min) | USP Number of plates | USP Tailing |
| Caffeine | 1.42 | 100.00 | 89307 | 268560 | 5566 | 1.24 |
Order Information
| P/N | Specification |
| 00240-11012 | Ultisil® UHPLC Amide， 1.8μm，2.1×100mm |
| 00274-11012 | Ultisil® UHPLC HILIC Amphion Ⅱ，1.8μm， 2.1×100mm |
Overview of 10 Ultra High-Pressure Bonding Phases Introduced by Welch Materials
Ultisil® Series
| Ultisil® UHPLC XB-C18 | The most versatile C18 column. |
| Ultisil® UHPLC HILIC | HILIC silica column. |
| Ultisil® UHPLC XB-C8 | Slightly lower retention capability than XB-C18, requires less analysis time for separation of neutral and other strongly retained compounds. |
| Ultisil® UHPLC XB-Phenyl | Phenyl column with complementary selectivity to C18, suitable for separation of compounds with aromatic rings. |
| Ultisil® UHPLC AQ-C18 | C18 column resistant to 100% aqueous mobile phase, suitable for separation of polar compounds. |
| Ultisil® UHPLC LP-C18 | Acid-resistant and resistant to 100% aqueous mobile phase C18 column, more polar than AQ-C18. |
| Ultisil® UHPLC Polar-RP | More water-resistant than AQ-C18, suitable for separation of polar compounds, no tailing observed in the analysis of basic substances. |
| Ultisil® UHPLC PFP | Pentafluorophenyl column, fluorinated + phenyl stationary phase, provides different selectivity compared to straight-chain alkane columns, suitable for separation of aromatic compounds, compounds with fluorine, halogens, and compounds without fluorine or halogens. |
Xtimate® Series
| Xtimate® UHPLC C18 | Organic-inorganic hybrid packing material, alkali-resistant C18 column. |
| Xtimate® UHPLC Phenyl-Hexyl | Organic-inorganic hybrid packing material, alkali-resistant phenyl column, hexyl-hexyl high-density bonding, more water-resistant and stable compared to regular phenyl columns. |
Welch Ultra High-Pressure Liquid Chromatography (UHPLC) columns offer a diverse range of bonding phases, allowing for faster detection times while meeting the requirements for result accuracy and separation efficiency of different analytical projects.
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