Sample preparation is the most time and labor-intensive step in the entire analysis process, accounting for approximately 85% of the analysis time. Therefore, it is essential to choose an efficient device to save time and effort.
HT4000E, a powerful tool for chemical analysis, combines SPE and HPLC injection capabilities. It is an all-in-one SPE-LC sample preparation workstation introduced by Welch Materials and Italy’s HTA through in-depth collaboration.
HT4000E can achieve online or offline SPE, with key features including ease of use, large sample capacity, application versatility, modular and scalable design, and laboratory automation readiness.
How does the HT4000E work?
Samples are processed sequentially: each sample can be processed using a multi-step method, providing the flexibility needed for complex processes. The HTAPREP software supports sample sequences. Therefore, if you need to process small batches of samples with different methods, the HT4000E makes sense as well.
A typical SPE method consists of different steps: Some of which may not appear in your routine laboratory operations. In order to better understand the working principle of the HT4000E, we provide a brief description of each step.
The sample rack is aligned with the waste rack of the SPE system. The activation solvent is aspirated from the nozzle and distributed into the SPE column; if multiple solvents are required, the task is repeated for the other solvents.
02 Sample loading
The syringe draws the sample from the sample tube/bottle and loads it onto the SPE column.
03 The washing solvent is aspirated from the nozzle and distributed to the SPE column.
The sample rack moves to the top of the elution rack. Elution solvent is drawn from the nozzle and distributed onto the SPE column, and the eluted sample is then collected in the elution bottle.
05 Drying of the column
If necessary in each step, the column can be dried with air using the injector.
Take an equal portion of the eluate from the elution bottle and inject it into the HPLC through the injection valve; at the same time, return the sample information to the HPLC for data acquisition in real-time. If necessary, preliminary derivatization steps can be performed in the elution bottle, or the sample can be transferred to a mixing bottle first.
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