Water Soluble Vitamin on Xtimate C18

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Sample

Gradient Program

Time(min)Mobile Phase A(%)Mobile Phase B(%)Flow Rate
010001.2
1310001.2
2801001.5
3810001.2
ColumnXtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043)
Mobile PhaseA: Accurately weigh 0.87g potassium dihydrogen phosphate, 0.41g potassium dihydrogen phosphate, dissolve with water and add acetonitrile 125ml, diluted with water to 1000ml, PH 7.40 B: Water/acetonitrile/phosphoric acid =499/499/2
Detection360 nm
Temperature40℃
Flow Rate0.5 mL/min
Injection Volume20 µL

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