Sample Detection Chromatogram
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm (P/N:00207-31043) |
| Detection | 360 nm |
| Temperature | 40 ℃ |
| Injection Volume | 20 µl |
Mobile Phase
Mobile Phase A: 0.87g dipotassium phosphate, 0.41g monopotassium phosphate, dissolve in water and add 125mL acetonitrile. Dilute with water to 1000ml, PH:7.40;
Mobile Phase B: Water- acetonitrile-phosphoric acid=499:499:2
| Time(min) | Mobile Phase A (%) | Mobile Phase B (%) | Flow Rate(ml/min) |
| 0 | 100 | 0 | 1.2 |
| 13 | 100 | 0 | 1.2 |
| 28 | 0 | 100 | 1.5 |
| 38 | 100 | 0 | 1.2 |
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