Water Soluble Vitamin on Ultisil AQ-C18

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Sample Detection Chromatogram

ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm   (P/N:00207-31043)
Detection360 nm
Temperature40 ℃
Injection Volume20 µl

Mobile Phase

Mobile Phase A: 0.87g dipotassium phosphate, 0.41g monopotassium phosphate, dissolve in water and add 125mL acetonitrile. Dilute with water to 1000ml, PH:7.40;

Mobile Phase B: Water- acetonitrile-phosphoric acid=499:499:2

Time(min)Mobile Phase A (%)Mobile Phase B (%)Flow Rate(ml/min)
010001.2
1310001.2
2801001.5
3810001.2

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