Water Soluable Vitamin on Ultisil AQ-C18
Sample
| Column | Ultisil®AQ-C18, 4.6×250mm, 5μm |
| Mobile Phase | Mobile Phase A: weigh 0.87g dipotassium phosphate, dipotassium phosphate 0.41g, dissolve with water and add 125 ml acetonitrile, dilute with water to1000ml, pH:7.40 Mobile Phase B: water-acetonitrile-phosphoric acid=499：499：2 |
| Wavelength | 360 nm |
| Temperature | 40℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 μl |
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