Water Soluable Vitamin on Ultisil AQ-C18

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ColumnUltisil®AQ-C18, 4.6×250mm, 5μm
Mobile PhaseMobile Phase A: weigh 0.87g dipotassium phosphate, dipotassium phosphate 0.41g, dissolve with water and add 125 ml acetonitrile, dilute with water to1000ml, pH:7.40
Mobile Phase B: water-acetonitrile-phosphoric acid=499:499:2
Wavelength360 nm
Flow Rate1 ml/min
Injection Volume20 μl

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