Vulcanizer on Topsil C18

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Blank Solution

ColumnTopsil C18, 4.6×250mm, 5μm (P/N 00410-01043)
Detection254nm
Temperature43℃
Flow Rate1.0ml/min
Injection Volume1ul

Mobile Phase

A: 0.1mol/L sodium acetate(pH6.5) : Acetonitrile = 93:7

B: Acetonitrile:Water=20:80

TimeA%B%
05050
202080
220100
350100
365050

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