Vitamin K1 in Dairy Products on Ultisil AQ-C18, Ultisil Zn

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Reference substance

 Vitamin K1 Standard Sample (Concentration 2 ug/mL)

 Total 2374589132731   
ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm  P/N: 00207-31043 Ultisil® Zn 4.6×50mm   P/N: 00225-51037
Mobile Phase900mL Methanol (4.6), 100 mL dichloromethane (4.7), 0.3mL glacial acetic acid (4.8), 1.5g zinc chloride (4.9), 0.5g Sodium acetate anhydrous (4.10), filter with 0.45μm membrane after dissolution
Detection243 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume20 µl

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