Ultisil XB-C30, 4.6×250mm, 3μm, Mixed Reference
| Column | Ultisil XB-C30, 4.6×250mm, 3μm (P/N 00223-21043) Ultisil XB-C30, 4.6×150mm, 3μm (P/N 00223-21041) Ultisil PFP, 4.6×250mm, 5μm (P/N 00224-31043) |
| Detection | 294nm/325nm |
| Temperature | 20℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10ul |
| Note | Separation is sensitive to column temperature(20℃) |
Mobile Phase
| Time/min | Methanol | Water |
| 0 | 96 | 4 |
| 13 | 96 | 4 |
| 20 | 100 | 0 |
| 24 | 100 | 0 |
| 24.5 | 96 | 4 |
| 30 | 96 | 4 |
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