Vitamin D3
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 25 | 100 | 0 |
| 26 | 0 | 100 |
| 30 | 0 | 100 |
| 31 | 100 | 0 |
| 37 | 100 | 0 |
| Column | Welch Ultisil SiO2 4.6×250，5μm (P/N: 00200-31043) |
| Mobile Phase | A: 0.45% isopropanol in n-hexane solution, B: 20.0% isopropanol in n-hexane solution |
| Detector | 265nm |
| Temperature | 25℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
| Notes | The silica column has a great influence on the water content in the reagent. |
If you have any problem or require further information, please contact info@welchmat.com.
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