Vitamin D3 Test Report on Ultisil SiO2

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Vitamin D3

Gradient Program

Time(min)A%B%
01000
251000
260100
300100
311000
371000
ColumnWelch Ultisil SiO2 4.6×250,5μm (P/N: 00200-31043)
Mobile PhaseA: 0.45% isopropanol in n-hexane solution, B: 20.0% isopropanol in n-hexane solution
Detector265nm
Temperature25℃
Flow Rate1ml/min
Injection Volume20ul
NotesThe silica column has a great influence on the water content in the reagent.

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