Vitamin D3 on Ultisil XB-C18
| Column | Ultisil XB-C18(II) 4.6×250 mm, 5 μm |
| Mobile Phase | Phosphate buffer: acetonitrile =92:8 |
| Detection | 265 nm |
| Temperature | 30°C |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 5 µl |
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