Vitamin B1 on Ultisil XB-C18

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Vitamin B1 standard working solution

ColumnUltisil XB-C18(4.6×250mm,5μm)
Mobile Phase0.05mol/L sodium acetate solution: Methanol=65:35
DetectionThe excitation wavelength is 375nm and the emission wavelength is 435nm
Temperature30°C
Flow Rate0.8 ml/min
Injection Volume20 µl

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