Vitamin A and E in Milk Powder

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Vitamin A, δ-tocopherol, γ- tocopherol 1mg/L, α-tocopherol 2mg/L

No.Peak NameRtArea Height AreaAsymmetryResolutionPlates
  minmAU*minmAU%(EP)(EP)(EP)
1VitaminA6.61933508406770.2801.07213593
2δ-tocopherol11.69442333088.8781.08316.82115447
3γ-tocopherol14.09939282308.2390.9925.77115227
4α-tocopherol15.648600931112.6031.0293.17614575
ColumnUltisil® PFP 4.6×250mm,5μm
Mobile PhaseA-Methanol,B-Water(GradientA:B=93:7)
Wavelength0-7.10min:325nm 7.10-10min:265nm
10-25min:294nm
Temperature20℃
Flow Rate0.8mL/min
Injection Volume5 μl

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