Valsartan and Amlodipine on Ultisil PFP

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ColumnWelch Ultisil PFP colunm(2. 1×50mm,5 μm)
 Detector254nm
Temperature40℃
Flow Rate0.6 mL/min
Injection Volume5 μL

Mobile Phase

TimeMobile Phase A (%)Mobile Phase B (%)
0-3.2053→4047→60
3.20-3.3040→060→100
3.30-3.700100
3.70-3.800→53100→47
3.80-4.505340

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