| Column | Welch Ultisil PFP colunm(2. 1×50mm，5 μm) |
| Detector | 254nm |
| Temperature | 40℃ |
| Flow Rate | 0.6 mL/min |
| Injection Volume | 5 μL |
Mobile Phase
| Time | Mobile Phase A (%) | Mobile Phase B (%) |
| 0-3.20 | 53→40 | 47→60 |
| 3.20-3.30 | 40→0 | 60→100 |
| 3.30-3.70 | 0 | 100 |
| 3.70-3.80 | 0→53 | 100→47 |
| 3.80-4.50 | 53 | 40 |
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