Partial scale of sample
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 5 | 100 | 0 |
| 32 | 87 | 13 |
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | A: 10 mmol/L potassium dihydrogen phosphate (pH adjusted to 3.50 with 10% phosphoric acid): acetonitrile = 980:20; B: acetonitrile; |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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