Urokinase Protein on Ultisil LP-C18

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Injection Volume 10 µl

ColumnUltisil® LP-C18, 5μm,300Å, 4.6×150mm    PN: 00208-33041
Detection280 nm
Temperature35℃
Flow Rate1 ml/min
Injection Volume10 µl, 15 µl, 20 µl

Mobile Phase

A: (0.1% TFA) (PH around 2.5) pure water B: Acetonitrile (0.1% TFA)

Time (min)A(%)B (%)
01000
302080
372080
381000
481000

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