Test sample 1ml/min，Welch Ultisil PFP (4.6*250mm, 5μm) acetonitrile-water = 40/60
| Column | Welch Ultisil PFP 4.6×250mm，5μm (P/N: 00224-31043) |
| Mobile Phase | Acetonitrile-water=40/60 |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10µl |
| Notes | The proportion of organic phase has a greater impact on separation |
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