C
| Column | Xtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043) |
| Detection | 210 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
Mobile Phase
A: 0.2% acetic acid Solution B: acetonitrile
| Time(min) | Acetonitrile (%) | 0.1% trifluoroacetic acid (%) |
| 0 | 0 | 90 |
| 15 | 60 | 40 |
| 15.1 | 10 | 90 |
| 25 | 10 | 90 |
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