Carotenoids is a broad class of natural products, of which over 600 types have been found so far, including compounds of different carbon chain length, such as C40, C50 and C30 etc. They are well known to have many biological functions, including cancer prevention and treatment functions.
Compared to classical C18 stationary phases, the C30 phase is much more hydrophobic and retaining. Even when pure organic eluent is applied, many sample solutes, such as carotenoids, are able to retain. Ultisil C30 is designed for the separation of geometric isomers, polar carotenes, polar and nonpolar xanthophylls, steroids, retinols and fat-soluble vitamins (A, D, K and E).
- Polymeric C30 alkyl chains
- Very lipophilic
- Exceptional selectivity pattern for geometric isomers
Column Parameters
Columns | USP | pH range | Carbon Loading(%) | Surface Area(m2/g) | Endcapped | Max. Temp.(°C) |
Ultisil XB-C30 | L62 | 1.5-10.0 | 22%(120Å) | 320(120Å) | Yes | 60(pH≤6.5), 40(pH>6.5) |
Applications
Separation of Carotenoids

Column | Ultisil XB-C30, 4.6×250mm, 5μm |
Mobile Phase | A: methanol / MTBE / water=81/15/4 B: methanol/ MTBE=10/90 |
Gradient Program | 0-90 min (0%B-100%B) |
Flow rate | 1.0mL/min |
Detector | 450nm |
Temperature | Ambient |
VE( α,β,γ,δ-tocopherol) and VA

Column | Ultisil XB-C30, 4.6×250 mm, 5μm |
Mobile Phase | A: water B: methanol |
Gradient Program | See PDF |
Flow Rate | 0.8 mL/min |
Detector | 294/325 nm |
Temp. | 20°C |
Injection Volume | 10μL |
Febuxostat Intermediate

Column | Ultisil XB-C30, 4.6×250 mm, 5μm |
Mobile Phase: | Acetonitrile/ water=70/30 |
Flow rate | 1.0mL/min |
Detector | 230nm |
Temp. | 30°C |
Injection Volume | 20μL |
Catalog Downloads
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About us
Welch Materials is a multinational company that develops and manufactures chromatography consumables including HPLC column, Solid Phase Extraction (SPE) column, GC column, Prep column, Flash column and packing materials.
Our initial strength was our extensive experience on particle surface modification science and techniques. We are experts on bonding
chemistry and innovative packing materials for chromatography applications. Through the optimal utilization of our resources, we have developed many innovative LC and GC consumable products, in particular, our six series HPLC columns, Ultisil®, Welchrom®, Xtimate®, Topsil®, Boltimate® and Blossmate®.
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