Carotenoids is a broad class of natural products, of which over 600 types have been found so far, including compounds of different carbon chain length, such as C40, C50 and C30 etc. They are well known to have many biological functions, including cancer prevention and treatment functions.
Compared to classical C18 stationary phases, the C30 phase is much more hydrophobic and retaining. Even when pure organic eluent is applied, many sample solutes, such as carotenoids, are able to retain. Ultisil C30 is designed for the separation of geometric isomers, polar carotenes, polar and nonpolar xanthophylls, steroids, retinols and fat-soluble vitamins (A, D, K and E).
- Polymeric C30 alkyl chains
- Very lipophilic
- Exceptional selectivity pattern for geometric isomers
Column Parameters
| Columns | USP | pH range | Carbon Loading(%) | Surface Area(m2/g) | Endcapped | Max. Temp.(°C) |
| Ultisil XB-C30 | L62 | 1.5-10.0 | 22%(120Å) | 320(120Å) | Yes | 60(pH≤6.5), 40(pH>6.5) |
Applications
Separation of Carotenoids
VE( α,β,γ,δ-tocopherol) and VA
| Column | Ultisil XB-C30, 4.6×250mm, 5μm |
| Mobile Phase | A: methanol / MTBE / water=81/15/4 B: methanol/ MTBE=10/90 |
| Gradient Program | 0-90 min (0%B-100%B) |
| Flow rate | 1.0mL/min |
| Detector | 450nm |
| Temperature | Ambient |
| Column | Ultisil XB-C30, 4.6×250 mm, 5μm |
| Mobile Phase | A: water B: methanol |
| Gradient Program | See PDF |
| Flow Rate | 0.8 mL/min |
| Detector | 294/325 nm |
| Temp. | 20°C |
| Injection Volume | 10μL |
| Column | Ultisil XB-C30, 4.6×250 mm, 5μm |
| Mobile Phase: | Acetonitrile/ water=70/30 |
| Flow rate | 1.0mL/min |
| Detector | 230nm |
| Temp | 30°C |
| Injection Volume | 20μL |
Catalog Downloads：Ultisil XB-C30
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