Carotenoids is a broad class of natural products, of which over 600 types have been found so far, including compounds of different carbon chain length, such as C40, C50 and C30 etc. They are well known to have many biological functions, including cancer prevention and treatment functions.
Compared to classical C18 stationary phases, the C30 phase is much more hydrophobic and retaining. Even when pure organic eluent is applied, many sample solutes, such as carotenoids, are able to retain. Ultisil C30 is designed for the separation of geometric isomers, polar carotenes, polar and nonpolar xanthophylls, steroids, retinols and fat-soluble vitamins (A, D, K and E).

  • Polymeric C30 alkyl chains
  • Very lipophilic
  • Exceptional selectivity pattern for geometric isomers

Column Parameters

ColumnsUSPpH rangeCarbon Loading(%)Surface Area(m2/g)EndcappedMax. Temp.(°C)
Ultisil XB-C30L62 1.5-10.0 22%(120Å) 320(120Å) Yes 60(pH≤6.5), 40(pH>6.5)


Separation of Carotenoids

ColumnUltisil XB-C30, 4.6×250mm, 5μm
Mobile PhaseA: methanol / MTBE / water=81/15/4
B: methanol/ MTBE=10/90
Gradient Program0-90 min (0%B-100%B)
Flow rate1.0mL/min

VE( α,β,γ,δ-tocopherol) and VA

ColumnUltisil XB-C30, 4.6×250 mm, 5μm
Mobile PhaseA: water
B: methanol
Gradient Program See PDF
Flow Rate0.8 mL/min
Detector294/325 nm
Injection Volume10μL

Febuxostat Intermediate

ColumnUltisil XB-C30, 4.6×250 mm, 5μm
Mobile Phase:Acetonitrile/ water=70/30
Flow rate1.0mL/min
Injection Volume 20μL

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About us

Welch Materials is a multinational company that develops and manufactures chromatography consumables including HPLC column, Solid Phase Extraction (SPE) column, GC column, Prep column, Flash column and packing materials.

Our initial strength was our extensive experience on particle surface modification science and techniques. We are experts on bonding
chemistry and innovative packing materials for chromatography applications. Through the optimal utilization of our resources, we have developed many innovative LC and GC consumable products, in particular, our six series HPLC columns, Ultisil®, Welchrom®, Xtimate®, Topsil®, Boltimate® and Blossmate®.