Carotenoids is a broad class of natural products, of which over 600 types have been found so far, including compounds of different carbon chain length, such as C40, C50 and C30 etc. They are well known to have many biological functions, including cancer prevention and treatment functions.
Compared to classical C18 stationary phases, the C30 phase is much more hydrophobic and retaining. Even when pure organic eluent is applied, many sample solutes, such as carotenoids, are able to retain. Ultisil C30 is designed for the separation of geometric isomers, polar carotenes, polar and nonpolar xanthophylls, steroids, retinols and fat-soluble vitamins (A, D, K and E).

  • Polymeric C30 alkyl chains
  • Very lipophilic
  • Exceptional selectivity pattern for geometric isomers

Column Parameters

ColumnsUSPpH rangeCarbon Loading(%)Surface Area(m2/g)EndcappedMax. Temp.(°C)
Ultisil XB-C30L621.5-10.022%(120Å)320(120Å)Yes60(pH≤6.5), 40(pH>6.5)


Separation of Carotenoids

VE( α,β,γ,δ-tocopherol) and VA

ColumnUltisil XB-C30, 4.6×250mm, 5μm
Mobile PhaseA: methanol / MTBE / water=81/15/4
B: methanol/ MTBE=10/90
Gradient Program0-90 min (0%B-100%B)
Flow rate1.0mL/min
ColumnUltisil XB-C30, 4.6×250 mm, 5μm
Mobile PhaseA: water
B: methanol
Gradient ProgramSee PDF
Flow Rate0.8 mL/min
Detector294/325 nm
Injection Volume10μL
ColumnUltisil XB-C30, 4.6×250 mm, 5μm
Mobile Phase:Acetonitrile/ water=70/30
Flow rate1.0mL/min
Injection Volume20μL

Catalog DownloadsUltisil XB-C30

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