Ultisil Ion Exchange columns and media are silica based strong anion exchange (SAX – ammonium-functionalized silane) and strong cation exchange (SCX – covalently bonded aromatic sulfonic acid moiety) columns – with high resolution and high efficiency.
- Organic modifiers such as acetonitrile and methanol may be used with SAX and SCX columns, within organic/buffer solubility constraintsVery lipophilic
- Retention can be controlled by varying pH, ionic strength and organic modifier content.
Column Parameters
| Columns | USP | pH range | Carbon Loading(%) | Surface Area(m2/g) | Endcapped | Max. Temp.(°C) |
| Ultisil XB-SAX | L14 | 2.0-8.0 | 7.5(120Å), 1.5(300 Å) | 320(120Å), 90(300 Å) | No | 60(pH≤6.5), 40(pH>6.5) |
| Ultisil XB-SCX | L9 | 2.0-8.0 | 12(120Å), 5(300Å) | 320(120Å), 90(300 Å) | No | 60(pH≤6.5), 40(pH>6.5) |
Applications
13 Heparin Disaccharides
| Column | Ultisil XB-SAX, 3.0 ×250mm, 5µm |
| Injection Volume | 10 µL |
| Flow rate | 0.45mL/min |
| Detector | 234 nm, 202 nm |
| Temperature | 50°C |
Chondroitin Sulfate
| Column | Ultisil XB-SAX, 4.6×250mm, 5µm |
| Mobile Phase | A: water, adjust pH 3.5 with diluted HCl B: 2 mol/L NaCl, adjust pH 3.5 with diluted HCl |
| Flow Rate | 1.0 mL/min |
| Detector | 232 nm |
| Temp. | Ambient |
| Injection Volume | 20μL |
| Mixed Standards: | Chondroitin disaccharide(B) 6- sulfated chondroitin disaccharide(C) 4- sulfated chondroitin disaccharide(A) |
Metformin HCL
| Column | Ultisil XB-SCX, 4.6×250mm, 5µm |
| Mobile Phase: | 1.7% NH4H2PO4( pH 3.0 adjusted by H3PO4) |
| Flow rate | 1.0mL/min |
| Detector | 218nm |
| Temp. | Ambient |
| Injection Volume | 10μL |
| Samples In Order | Icyandiamide, melamine, metformin HCL |
Orazamide
| Column | Ultisil XB-SCX, 4.6×250mm, 5µm |
| Mobile Phase: | 1.0% NH4H2PO4( pH 3.0 adjusted by H3PO4) |
| Flow rate | 1.0mL/min |
| Detector | 215nm |
| Temp. | 30°C |
| Injection Volume | 10μL |
| Samples In Order | Orotic acid, Pyridinepropanimidamide, 4-Amino-5-imidazolecarboxamide hydrochloride and AZO |
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About us
Welch Materials is a multinational company that develops and manufactures chromatography consumables including HPLC column, Solid Phase Extraction (SPE) column, GC column, Prep column, Flash column and packing materials.
Our initial strength was our extensive experience on particle surface modification science and techniques. We are experts on bonding
chemistry and innovative packing materials for chromatography applications. Through the optimal utilization of our resources, we have developed many innovative LC and GC consumable products, in particular, our six series HPLC columns, Ultisil®, Welchrom®, Xtimate®, Topsil®, Boltimate® and Blossmate®.
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