ColumnUltisil LP-C18 Separate FP and its chiral isomers
Mobile PhaseMobile phase A: 0.01mol/L sodium dihydrogen phosphate solution (containing 10g/L sulfonyl-β-cyclodextrin sodium salt, adjust the pH value to 2.0 with phosphoric acid
Time Mobile Phase A Methanol (%)
0-20 75-65 25-35
20.1-25 75 25
Detection205nm
Temperature30℃
Flow Rate0.8ml/min
Injection Volume10 μL (high concentration standard mix); 20 μL (low concentration standard mix)

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