| Column | Ultisil LP-C18 Separate FP and its chiral isomers |
| Mobile Phase | Mobile phase A: 0.01mol/L sodium dihydrogen phosphate solution (containing 10g/L sulfonyl-β-cyclodextrin sodium salt, adjust the pH value to 2.0 with phosphoric acid Time Mobile Phase A Methanol (%) 0-20 75-65 25-35 20.1-25 75 25 |
| Detection | 205nm |
| Temperature | 30℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10 μL (high concentration standard mix); 20 μL (low concentration standard mix) |
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