Test Sample

ColumnWelch Ultisil XB-C18 4.6×250mm,5μm (P/N: 00201-31043)
Mobile PhasePhosphate buffer (0.1 M / L sodium dihydrogen phosphate solution, adjust pH to 4.4 with phosphoric acid)- Acetonitrile = 80:20
Detector254nm
Temperature30℃
Flow Rate0.5ml/min
Injection Volume10ul

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