Test Sample
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | Phosphate buffer (0.1 M / L sodium dihydrogen phosphate solution, adjust pH to 4.4 with phosphoric acid)- Acetonitrile = 80:20 |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 0.5ml/min |
| Injection Volume | 10ul |
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